Trying to carryout hydrogen peroxide scavenging activities of oily extracts not miscible in water. However, I will like to know if there is any chance that DMSO when used in preparing the test extracts will affect the result. Thanks
Some papers suggest that it have negligible level of effect on the antioxidant activity. For more detail insights, please read this Ms Article A high correlation indicating for an evaluation of antioxida...
Thanks
Hi David,
I will advise you to prepare a stock concentration of your sample in 1000X. If the highest working concentration is 100ug/ml, multiply by 1000 to have a 100 mg/ml stock concentration in 100% DMSO. It will help you to have about 0.1% DMSO in the final reaction mixture.
I've undergone this issue when I tried to test some phyto-compounds isolated from the butanolic fraction of a plant extract. I tried to use DMSO instead of MeOH or EtOH because they could only be dissolved in DMSO.
Interestingly, the DMSO did not affect the results but it really delayed the activity of the antioxidant compounds by at least 48 hours.
I think it may be due to the ability of DMSO to stabilize the radical.
In in vivo antioxidant activity tests with C. elegans, DMSO was used as a target in the culture media and no significant effect was obtained.
I suggestcan use DMSO as blank control , and then minus the absorbabce value of blank DMSO from DPPP+sample + DMSO absorbance value
I suggestcan use DMSO as blank control , and then minus the absorbabce value of blank DMSO from DPPP+sample + DMSO absorbance value
I suggestng and try by can use DMSO as blank control , and then minus the absorbance value of blank DMSO from DPPH+sample + DMSO absorbance value, 0.1% concntration of DMSo
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