Dyslipidemia and erectile dysfunction.

More Harasit Kumar Paul's questions See All

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

How to scrape off the surface layer of compound sputtering targets as with multiple usage top layer's stoichiometry changes from the original target?

I am experiencing this for the Bi2Se3 sputtering target. Initially, even if the target was 2:3 in Bi : Se, with a few sputtering runs, due to the high escaping tendency of selenium, the top layer...

23 July 2024 1,649 1 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

What are the best ways to automate my job and live without working?

I want to know the best methods to automate my job and live without working?

12 July 2024 5,993 2 View

What are the best ways to earn more money as a researcher?

I would like know what are the best ways to earn more money as a researcher?

11 July 2024 4,695 2 View

How do I monitor my CCTV camera in Bangalore from California?

I would like to know how do I monitor my CCTV camera at my home in Bangalore from my current location which is California in USA?

11 July 2024 831 0 View

How do I check if the research work is human made or AI generated?

I would like to know how to check if a given research work is human made or AI generated?

11 July 2024 8,645 5 View

How does an AI detection tool work?

I am interested in knowing how does an AI detection tool work and what's the main logic behind it? How it is able to detect the presence of AI work?

11 July 2024 3,889 6 View

How do I prove that my research work is noval and not plagiarized?

I would like to know how to prove my research work is new and not copied from other people's work or AI tool?

09 July 2024 8,235 2 View

Why does the male need for intercourse dominate any assessment of women's sexuality?

According to some scholars, the allegedly high prevalence of female sexual dysfunction has to do with the fact that the standard of ‘normality’ is based on the male sexual response. Krasnow &...

03 July 2024 10,101 2 View

How to insert cholesterol inside DPPC membrane by replacing same number of DPPC molecules in GROMACS?

I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...

30 June 2024 4,160 2 View

What is the best way to calculate the weight of each lipid component needed to achieve the desired molar ratios?

I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...

26 June 2024 1,135 0 View

Why is Femlae Sexual Dysfunction so common if female orgasm is the norm?

One of the diagnostic criteria for FSD (female sexual dysfunction) is feeling distressed. But what causes the distress? Is it the condition itself, or is it what you think is expected of you and...

07 June 2024 1,371 0 View

Can delirium cause brain damage that is similar to damage caused by a stroke?

Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...

07 June 2024 8,723 2 View

Separation of Cholesteryl esters from alkanes/alkenes?

Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...

02 June 2024 5,545 0 View

SH-SY5Y total cholesterol?

I'm currently doing total cholesterol assay in SH-SY5Y cells treated with 0, 25, 50, 100ug cholesterol/mL (using cholesterol:mbcd, sigma) Lipid extraction is done by chloroform and methanol. After...

12 April 2024 9,353 3 View

Purification of Apolipoproteins from plasma?

Anyone have experience with this? I am trying Dextran sulfate/manganese to get at LDL and VLDL first. But the literature out there is confusing. Would appreciate some advice on the protocol if...

02 April 2024 5,225 1 View

What test determines the LDL particle size?

If the triglyceride HDL ratio is normal is the LDL particle size big?

10 February 2024 4,145 3 View

How can we detect Cholesterol from a liposomal formulations? How can we separate Cholesterol and Phospholipids in liposomal formulation?

Separation techniques for two miscible liquids.

22 November 2023 671 1 View