Anyone have experience with this? I am trying Dextran sulfate/manganese to get at LDL and VLDL first. But the literature out there is confusing. Would appreciate some advice on the protocol if anyone has tried it.
Thanks in advance
Purification of apolipoproteins from plasma involves several steps to isolate and enrich these proteins. Apolipoproteins are the protein components of lipoprotein particles, which transport lipids such as cholesterol and triglycerides through the bloodstream.It's important to note that the specific methods and conditions for purifying apolipoproteins may vary depending on factors such as the source of plasma, the target apolipoprotein, and the available equipment and expertise in the laboratory. Additionally, precautions should be taken to minimize protein degradation and maintain sample integrity throughout the purification process.