Electroporation requires optimisation, you might need to change voltage and other parameters, and it not necessarily more effective then chemical transformation. It all depends on the cell type you are using.
Electroporation requires optimisation, you might need to change voltage and other parameters, and it not necessarily more effective then chemical transformation. It all depends on the cell type you are using.
I guess your electrocompetent cells are not good enough or your electroporation conditions are not ideal. For instance, do your sample contain too much salts? If so, electroporation can be severely affected. Are you cleaning-up your samples and leaving them in water before electroporation?
How are your time contants (in ms) after electroporation? Are you having arch? If so, a message should appear in the display and you should notice a noise (like a small explosion).
In general, electroporation must be more efficient if everything is ok. Which is your strain? What is the transformation efficiency of your cells?
Hi Flora,
Electroporation is generally more efficient than chemical transformation, however as mentioned above , it needs optimization of the parameters, which may further vary with different strains. SO without doing these requisites, it is not possible to make a statement about relative efficiency of two protocols
bets regards
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