·Prepare a 10% solution of skim milk and autoclave it.
·Grow fungal culture on suitable medium till
sporulation.
·Add 2 or 3 ml of sterile skim milk to the culture. Gently
scrape the surface with a sterile loop to loosen the
spores.
·Add 0.5 ml of the milk-spore (0.5 m1/4 g) suspension to
a vial of chilled, sterilized soil. Add the suspension
slowly and evenly.
Adding sterile skim milk to the culture could serve as a natural balanced emulsifier for spores, I guess!
it increase the survival rate of the spores due to high protein concentration.
Dear Priyanka,
In addition to my previous answer: Skimmed milk /glycerol mixture (protective solution) is believed to offer good protection to the spores against freezing and thawing damage.
Please see the attached article.
All the best
Mohammed
Thank you all for your kind reply...
The answer and publication given by Mohammed saleem Ali-shtayeh and Itzhack Polacheck is reasonable... thank's again in helping me in my research work...
With regards,
Priyanka Roy.
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