Hello Alice, Thanks! I actually used the recipe from NEB. Here is the link: https://www.neb.com/products/b0363-rna-loading-dye-2x. My adapted recipe is as follows: 485 uL of RNase free H20, 480 uL of >99.5% formamide, 25 uL of 0.5 mM EDTA (I made a 2mM EDTA in 1mL RNase free H2O first and added 10 uL of B-ME), 10 uL of 0.01% SDS and Bromophenol Blue (I made 1% SDS and Bromophenol Blue in 1 mL of RNase free H2O first). It says you can use it for both denaturing and non-denaturing gels. For non-denaturing gels, you just don't heat the samples. To be safe I kept my samples on ice after adding the loading dye. At first I was a bit suspicious as it contained formamide. However, I ran my first gel yesterday using the loading dye and it didn't denature my dsRNA.