Is the heat treatment in open atmosphere an effective method for decrease carbon on surface graphitic cast iron?

The target is removing or decreasing free carbon on the external surface of gray and ductile iron.

09 August 2020 612 2 View

I want an open source FDTD for SAR signal correction due to propagation in ionosphere?

SAR signal processing software or matlab code for SAR signal processing specially for SAR signal corrections due to propagation in ionosphere

26 March 2020 5,855 3 View

Why Researchers Dissolve Sodium Azide in Phosphate Buffer (0.1M) in mutagenasis?

Really i have three questions, if i can get answers about it! 1- First One, Usually researchers used Sodium Azide with concentrations (1-5 mM or 0.01-0.1%) with long time (15-20 hour) on pH=7, or...

07 October 2019 9,045 2 View

How can I store stool specimen for Molecular diagnosis for human parasites Is there a difference of storing for PCR or real PCR or multiplex ?

Molecular stool

08 January 2019 8,186 3 View

What is the best product size of SYBR Green Real-Time PCR to amplify c-DNA?

04 August 2016 2,127 9 View

Does SAHA or 5-aza increase the expression of housekeeing genes?

Hi all, I used ELISA for analyzing the expression of specific protein with treatment of epigentic modifiers (SAHA or 5aza). And I found that the expression of GAPDH became increased with treatment...

23 July 2016 9,193 1 View

Why add AMPure beads to DNA with adaptor in RAD Seq.?

i work on tomato genome with RAD Seq. and i want to know why we have to add AMPure beads to DNA

17 March 2016 4,698 3 View

How can I perform CCA (Canonical Correspondence analysis) with shading each locality on the CCA chart by means of CANOCO 4.5?

18 September 2015 4,485 2 View

Why I get a blank signal on western blot?

Hi, I am new to western blotting and so facing the problem of blank blots. i want to assess the expression of two membrane bound proteins, 45 & 74 KD, in rat heart tissue. My tissue samples...

07 September 2015 9,903 13 View

What is the best statistical test predict the effect of more than two independent categorical variables on a dependent categorical variable?

07 July 2015 1,836 9 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View