Dear colleagues, good night!
I'm in trouble with how I will present my results from the antioxidant activity from an Hibiscus extract.
In fact, I used the Brand-Williams protocol for DPPH inhibition assay and I'm with some doubts.
First of all, I would wanna know if i have to consider the dilution of the extracts samples in the DPPH medium used during the assay. For example: if I have dissolved my extracts for concentrations ranging between 2000-125 micrograms/mL and I put 0,3 mL of these solutions together with 3 mL of DPPH, so I have to consider my extracts concentrations between 50-3,125 micrograms/mL (final concentrations after dilution)?
Best wishes.
No. as long as you use other antioxidant as relative standard and similar treatment for it
Ashadi, I have always told my students and others writing up results of a study to examine the published literature and learn from it how information is reported and how investigators carry out their studies. In the case of the DPPH assay, there are literally thousands of papers reporting results using this assay. Read at least a couple of dozen papers using it and you'll have your answer.
You can try several times, or many times in laboratory with several different concentraions to find the best results. The high antioxidant activity is indicated by the change of DPPH colour from purple to yelllow. BHT 200 ppm as standard antioxidant also neccesary to use together with the sample.
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