In double immunoflourescence experiment by confocal, I intend to use two conjugated anti-cd11b and anti-f4/80 Ab (PE and FITC) in mice liver sections. I need your opinion whether,
1) Conjugated Ab with R-PE could be used with FITC?
2) In case of conjugated Ab, is it possible to use two Ab from single host that from Rat?
I am sorry that it is out of my area of expertise. I can not provide you the answer.
If your antibodies are directly labelled then it should not matter that they are both rat antibodies.
Hello
In links here you will find the Protocol .
Good Luck
http://www.biotechniques.com/multimedia/archive/00074/CRI-FP-Microscopy_74545a.pdf
http://www.ihcworld.com/_protocols/general_IHC/immunofl_double_squential.htm
http://www.abcam.com/ps/pdf/protocols/double%20immunofluorescence%20-simultaneous%20protocol.pdf
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