What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

How do anthropogenic pressures influence and will influence coastal groundwater according to the climate and its evolution?

Groundwater is of utmost importance in Haiti. It is the main source of drinking water for the Haitian population. The degradation of the quality of this resource is a major issue in the context...

29 June 2020 8,626 6 View

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Whether for a dissertation, a thesis or any other research work, there will be a problem in which it is clearly posed what is causing the problem and which imposes on the researcher the need to...

29 June 2020 4,902 9 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

How to purify large amounts of plasmid after restriction digest?

03 November 2019 2,686 4 View

What is the suitable and updated seismic attenuation models for Japan/Philippines?

We are working on different seismic studies both for research use and consultancy works. We've learned that Fukushima and Tanaka attenuation models are already outdated (model we used before)....

17 December 2018 7,970 2 View

Data of Propane/R290 and IsoButane/R600a Solubility in POE lubricant oils?

We need the test data of propane/R290 and isobutane/R600a solubility in POE lubricant oils. The data will be used to model the refrigerant quantity dissolved in compressor oils ( www.refriglab.com...

20 May 2018 9,451 1 View

How to choose between publishing and patenting?

Generally, academic researchers focus on publishing on public journals while company researchers are more interested in patenting. Sometimes, it is hard to choose between publishing your good...

20 April 2018 5,585 8 View

Shall we publish on online journals?

Most of us have the experiences being invited to write or review a paper for a new online journal. It looks that it is generally much easier and quicker to publish on the new online journals. Do...

19 April 2018 807 5 View

How can we refrigeration and air-conditioning professional benefit more from Cloud technology?

The cloud technology features its computing and storage capacity, and high reliability. The question is how we refrigeration and air-conditioning (RAC) professionals use the cloud computing, and...

26 February 2018 5,340 2 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View