I am working on DNA damage evaluation in stem cells
The first few qPCR runs the primers worked and I was getting signals in my samples/positive controls. However, now when I run another qPCR with the same primers I get no signal. I've tried using a...
17 October 2022 746 5 View
I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is...
17 October 2022 9,072 3 View
XRD data sheet standard JCPDS manual
01 February 2020 7,466 3 View
I am looking for EEG dataset for anesthesia, please where can I find such dataset?
10 November 2019 6,786 3 View
I’ve found out several clustering algorithms such as chameleon or even Spectral Clustering. its work to converting data to a weighted. KNN graph based on the distance between points. I'd like to...
09 October 2019 6,802 4 View
Scientific Workflow scheduling in cloud computing and I need suggestions on the gaps in this domain. The gaps that would need to be directions for future works?
08 September 2019 4,527 2 View
HI RG colleagues, some statistics' comments believe that the Baron And Kenny 1986 mediation method out of date. they suggested new effective approaches in term of testing the mediating variable.
07 August 2019 4,191 15 View
The advantages of this index are evident. The h-index can obviously be applied to any set of papers. Since the h-index cannot exceed the umber of underlying papers, many authors...
06 July 2019 6,316 3 View
The number required by the journal for the publication of articles does not exceed six thousand words, while the total words of article nearly 8000.. Should I follow the journal instructions in...
06 July 2019 7,211 33 View
Although I realize the success made using Python for different aspects of ANN and deep neural networks. However, I am a MATLAB user. Is it worth it to learn Python for that purpose? or is MATLAB...
04 May 2019 8,500 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
