I have this on-going project research where I have to determine the concentration of Endocrine-disrupting chemicals (EDCs) specifically on 4-octylphenol and 4-nonylphenol on bivalve.
1) I have referred previous studies where they used a variation of dry weights (etc: 0.5 g, 1.0 g, and 2.0 g) in bivalve to extract the compounds. Which one is the best weight to use and why? Does the weight size affect the concentration of the compounds inside the sample either to be easy to trace or not?
2) If most of my bivalve's size sample weighted approximately 0.1 g which lower than the previous studies, can I still proceed with extraction by using wholly 0.1 g of size sample?
I hope you can help me with this question.
Dear Mohamad
I am not sure if I completely understand your question. Any analysis method has its pros and cons. HPLC/LC-MS has the great advantage of enabling specific compound analysis based on molecular weight and composition, provided you have the right standards and your sample is not too polluted. You will need use the right extraction procedure for the the specific compound and column, obviously. Then, the quality of the result much depends on the concentration of the compound in your sample, not of the weight. I assume you use whole mussel flesh and not specific parts such as stomach or gonads? You may need to use several specimens to get enough amount of compounds, you have to be well above the detection limit to get a good quantification.
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