I am extracting a high molecular weight organic compound from biomass. I have been using petroleum ether (60 - 80oC). When I tried the same extraction using petroleum ether of grade; 40 -60oC., I did not get the expected result.
How about the compounds?it is thermal labile? Might be your compound also sensitive to the temperature
We have been making studies using Iron (III) Chloride and Copper (II) Chloride. It was observed that the pH of Iron (III) Chloride (0.03 M) is much lower than Copper (II) Chloride (0.03 M). We...
01 April 2015 4,918 9 View
We did a reaction and got a product that has higher molecular mass than the starting material. But, the melting points we got from the DSC analysis is indicating that the starting material which...
12 January 2015 7,113 4 View
I was doing a reaction involving these two compounds in the presence of a base and I found that the trimethyl aconitate was deprotonated by the base against 2,4-pentanedione. I was expecting...
11 January 2014 6,630 4 View
Wish to use the response factor in determining the purity of my compound.
05 July 2013 1,683 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View