Ultraviolet (UV) and antibiotics are widely used in laboratory. Does UV has negative effect to antibiotics？e.g. When disposing LB medium with ampicillin resistance, whether ultraviolet lamp sterilization can be continued after adding antibiotics?
If you mean while dispensing the media (with antibiotics) the lamp should be off, I would say, Yes it should be off. Not due to its harmful effect on antibiotics, but to avoid harmful effect of UV on skin. And if you are working in a clean bench, it is not required to have UV lamp on. UV lamp is just used to sterilize the clean bench.
Abhijeet Singh Just a guess : ). The clean bench is clean, but the antibiotics is taken from outside, and in extreme situations bacteria can stick to it. If UV has no harmful effect on antibiotics, I can turn on the UV lamp for few minutes after I leave to sterilize the completed media (with antibiotics) again. Or, I can put the antibiotics on the bench before alcohol and UV sterilization, and then turn off the UV lamp and dispensing the media without worried about the negative effect of UV on the antibiotics. Aha...forgave my poor English description.
Back when I was a student I was told that I should not expose non-defined media (i.e. containing complex constituents such as peptones and hydrolysates) to UV because growth promotion was affected due to the generation of reactive radicals. I have never found hard data backing this up, but these two papers suggest that medium composition may change and thus that caution is warranted if you want to irradiate antibiotic-containing media:
Article The effect of ultraviolet radiation upon yeast culture media
Article Treating Cell Culture Media with UV Irradiation Against Adve...
Hope it helps!
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