Does this melting curve imply incorrect qPCR results?

More Arash Azimzadeh-Isfanjani's questions See All

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Is this Melt Curve accurate?

Dear friends, The picture in the link below shows Melt Curve for the primer I have for the miRNA I am working with. This is the best set up that can accur for all 40 cDNAs. I myself think it is...

12 January 2014 3,150 6 View

Does this Melt Curve imply incorrect qPCR?

This is the best set up for the primer I have for the miRNA I am working with. It is same for all 40 samples. I myself think it is not applicable but, I need your comments. Thank you all.

11 January 2014 5,575 7 View

Does this Melt Curve imply incorrect qPCR?

This is the best set up for the primer I have for the miRNA I am working with. It is same for all 40 samples. I myself think it is not applicable but, I need your comments. Thank you all.

31 December 2013 2,949 8 View

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Dear friends, The picture in the link below shows Melt Curve for the primer I have for the miRNA I am working with. This is the best set up that can accur for all 40 cDNAs. I myself think it is...

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Primer contamination

Is there any useful remedy to use primer stock contaminated with DNA or cDNA?

06 July 2013 1,637 17 View

Which one should be used to draw a standard curve in qPCR, cDNA or PCR product of cDNA?

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Irina Afonina

Try to run your products on the gel. If you get one specific band for all of your samples, then I guess your melting curves are ok.

Norbert Bittner

I think you have a second product indicated by the curve at the lower temperature.. Can be primerdimers.. As Irina suggested run a agarose gel