7 Questions 5 Answers 0 Followers
Questions related from Arash Azimzadeh-Isfanjani
Dear researchers; A question arose for me about Real-Time analysis. I would appreciate you to comment about the question. For expression analysis we need a housekeeping gene to normalize Ct...
01 January 2019 9,041 4 View
I'm going to study the expression of a particular micro-RNA in tumor samples in comparison to normal tissue. We obtain gastric tumor and normal samples from same patient. How many samples do we...
04 April 2015 8,460 3 View
Dear friends, The picture in the link below shows Melt Curve for the primer I have for the miRNA I am working with. This is the best set up that can accur for all 40 cDNAs. I myself think it is...
01 January 2014 7,738 6 View
This is the best set up for the primer I have for the miRNA I am working with. It is same for all 40 samples. I myself think it is not applicable but, I need your comments. Thank you all.
01 January 2014 2,995 8 View
This is the best set up for the primer I have for miRNA I am working with. This curve happens for all my samples. I myself think it is not applicable. I need your comments. Thank you all.
01 January 2014 2,192 2 View
Is there any useful remedy to use primer stock contaminated with DNA or cDNA?
07 July 2013 1,679 17 View
I'm doing my my thesis on miRNA differential expression. A question arose when preparing for standard curve, should cDNA or its PCR product be used? When can we use each of them? Another question...
06 June 2013 6,799 3 View
