i use ethyl acetat and ethanol as solvent for extraction fruit of mangrove Avicennia marina. I have a problem with crude extract from ethanol solvent contain liquids that are very similar like oil. I did DPPH test from the extract of both solvents, and the results for ethanol have very weak activity IC50 = almost 2000 µg/mL. Does the liquid like oil affect the value of the activity obtained?
Yes according to the the solvents of extraction amount of antioxidant compunds and total antioxidant power also will changed. Polar solvents may increased phenolic compounds and it is related metsbolitis and lead to an increase of antioxidant power.
Removal of oily mater will changed your results it mask other compund fractions of ethanol crud extracts will have more power than crud extract it self
Yes, Antioxidant activity and extract yield strongly depend on the nature of extracting solvent and method. Different antioxidant compounds have various chemical characteristics and polarities that may or may not be soluble in a particular solvent.
Yes, it's depends on which solvent has polarity respect with taken sample. Each crude has various antioxidants form of secondary metabolites
yes it is effect on antioxidant activity by many way......
these articles have a good informations about your question
http://www.jlr.org/content/22/5/763.full.pdf
https://www.ocl-journal.org/articles/ocl/pdf/2001/01/ocl200181p89.pdf
Dewi Asri Anggraini Namrata Dwivedi Priti Gupta Mohammed Ismael Rushdi
Hi Dr. if the crude extract contain poly phenoles act as antioxidant
Yes, there are several studies shown that the type of crude extract affects the value of antioxidants
A Comparative Review on the Extraction, Antioxidant Content and Antioxidant Potential of Different Parts of Walnut (Juglans regia L.) Fruit and Tree
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600437/
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