Dear Scientists,
I performed RNA extraction of Fusarium oxysporum using TriZol method. I got the gel electrophoresis result. I suspect contimation with DNA. How can I interprete of this result?
Hello
Since you have heavy bands/smears above your ladder, looks like you have contamination with genomic DNA. Can you please specify the largest fragment size in your ladder? You may repeat your electrophoresis with 80% less extracted RNA to have a better image.
Good luck
Dear Professor Javadmanesh,
Thank you for your explanations.
The largest fragment size in my ladder seems to be 4 Kb. Please find attached the ladder fragment size.
You'll need to use DNase to avoid those contaminations and run properly further experiments since DNA can interfere in results analysis.
fred
you need to use DNase to eliminate DNA in your RNA sample. your gel image looks like your RNA was not alone :D
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