Does the RIN number have importance in NGS analysis?

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Carina Osterhof

It depends. Did you extract only the miRNA or the total RNA? If it's a micro RNA specific kit, a low RIN is to be expected, since it will filter out most of the ribosomal RNA that is required for the analysis. If you extracted total RNA, it could mean that your samples are degraded and this could then influence your downstream analysis.

Sreedevi M J

Thank you Ma'am

Thank you, Ma'am.@Carina Osterhof