Sreedevi M J

6 Questions 13 Answers 0 Followers

Questions related from Sreedevi M J

I didn't get any accurate results from my rtpcr, what is the reason?

I performed RT-PCR to validate my differentially expressed microRNAs. But I didn't get any accurate results. What is the reason? I also performed standard PCR (with a gradient temperature) to...

17 March 2024 8,865 4 View

How do we check the working of designed primers of micro RNA?

How do we check the working of designed primers of micro RNA? I have designed primers for my miRNA sequences. I ran normal PCR to check the working of primers, but I couldn't see any bands from my...

02 February 2024 786 7 View

Which Reference gene is best for the normalization of micro RNAs in qRT-PCR?

I want to know which reference gene is best for the normalization of micro RNAs in qRT-PCR. My research plant is coming under the family Leguminosae. Is there any plant or family-specific...

12 January 2024 5,230 3 View

Which tool is best for the synthesis of Gene specific real time PCR primers for micro RNAs?

I want to synthesize Gene-specific real-time PCR primers for my differentially expressed novel and conserved micro RNAs in plants.

22 December 2023 5,061 3 View

Does the RIN number have importance in NGS analysis?

I have to sequence miRNA from my research plant (common bean), and I got an RIN number between 5 and 6. Does this affect the NGS analysis?

06 October 2023 6,985 3 View

Could you please suggest a suitable kit for miRNA to cDNA conversion?

I want to convert micro RNA to cDNA (plant sample). Kindly recommend a suitable kit

16 May 2023 6,624 4 View