@Michael thanks for your reply, i mean if i will dilute the hrp-hapten conjugate in pbs so does the concentration (o.o1m or 0.005m) of pbs effect the result?
No, usually the buffer concentration has no significant effect. However, the dilution of HRP conjugates might lead to deactivation or adsorption of the conjugate to surfaces. Therefore, many researchers use PBS with bovine serum albumin (BSA, e.g 0.1%) or specific HRP diluents to avoid this.
@michael thank you so much for your reply, can you suggest me any paper related to the protocol to conjugate Tetracycline-HRP?
You do not need a general protocol for tetracycline HRP conjugation, you need a protocol, which is analogous to the immunoconjugate. Otherwise, your conjugation will work, but the conjugate will not work in your ELISA. How did you get the tetracycline antibodies?
@michael ok thank you so much for your response, i understand what you mean.
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