How to dissolve Pentachloronitrobenzene?

Pentachloronitrobenzene is used to prepare the "Nash & Snyder" media to isolate the Fusarium. But this is not dissolving in 75% ethanol and distilled water (Normal and hot both). So Please...

05 February 2021 687 1 View

How monolayer graphene can be grown on copper foil via LPCVD technique?

Please tell me about the rate (in SCCM unit) of the flow of gases and pressure inside the tube.

12 October 2020 8,315 3 View

If we cross Diploid plant (Female Parent) x Tetraploid plant. How much chances of getting viable seed?

In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?

16 June 2020 9,317 3 View

Is there any mathematical model to study the electric fringe field for parallel plates having equal and opposite potential?

Some Mathematical expressions will be helpful.

26 April 2020 8,000 2 View

Which form of polyaniline (emeraldine base or emeraldine salt ) show antibacterial property and why?

The emeraldine form of polyaniline often refer to as emeraldine Base (EB) is neutral, if it is doped (protonated) with acid(1M HCl) it is called emeraldine salts (ES) is conducting, with imine...

24 February 2020 5,117 3 View

Is there need to downscale CORDEX (RCM) data for our region? or Can we directly apply them after bias correcting them?

I have a query regarding data collected from CORDEX models and CMIP5 models. After reading about both, I have concluded that data from CMIP5 have to statistical downscale first then can apply for...

22 December 2019 2,655 5 View

How to perform a meta-analysis for single group?

How to perform a meta-analysis for single group? Single group means you don't have a comparison group. You have estimates i.e. Mean+_SD with sample size OR events and sample size for one...

11 August 2019 5,790 14 View

What is relation between impact factor and NAAS rating?

Many journals mention NAAS rating, but their Impact Factor is not mentioned, can we use one score to calculate the other. Sometimes impact factor given but NAAS rate is not given. so how to...

14 August 2018 1,679 17 View

How to analyse continuum ratio data (frequency) in SEM via AMOS?

I need to know, how to analyse in AMO during SEM?

09 April 2018 1,698 3 View

How should optimize my number of observation for my research work?

I have to analyze thermal stability of a coating for 4 different temperature for 4 different time interval. It is performed on different sample for calculation of various coating properties such...

06 February 2018 5,674 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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02 March 2021 3,060 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View