Sachin Kannangara Arachchige

3 Questions 5 Answers 0 Followers

Questions related from Sachin Kannangara Arachchige

Does spinning down DNA (in elution buffer) cause it to pellet at the bottom?

Hi, I have a question regarding DNA extractions. I follow a magnetic bead based protocol for DNA extraction and at the end of the protocol, there is a step to spin down the DNA samples which are...

22 December 2024 9,547 1 View

Heterozygosity detection via sanger sequencing?

Hello, It is my understanding that you can detect heterozygosity by looking for double peaks (see attached image) in sanger sequencing chromatograms. I have obtained sanger sequencing...

10 February 2019 5,289 7 View

Sangar sequencing failure?

I performed a PCR with a gradient of temperatures and ran the gel electrophoresis. There were bands present at each of the temperatures (lets say 59 and 55 degrees) I received the sangar...

01 February 2019 1,248 11 View