Sachin Kannangara Arachchige

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Questions related from Sachin Kannangara Arachchige

Heterozygosity detection via sanger sequencing?

Hello, It is my understanding that you can detect heterozygosity by looking for double peaks (see attached image) in sanger sequencing chromatograms. I have obtained sanger sequencing...

10 February 2019 5,223 7 View

Sangar sequencing failure?

I performed a PCR with a gradient of temperatures and ran the gel electrophoresis. There were bands present at each of the temperatures (lets say 59 and 55 degrees) I received the sangar...

01 February 2019 1,135 11 View