For my project, I need to use CD8+ T cells and follow exhaustion protocol. There are usually kits available with negative selection to obtain untouched CD8+ T cells.
Due to the way my experiment is planned, I need to use the fraction that is not T CD8 + T cells to isolate another cell type in the same patient, which would already be labeled after isolating CD8+ T cells. Unfortunately because of all the steps I have to go through to get the other cells, I could not use previously labeled cells.
I wonder if anyone has used positively selected (labeled) CD8+ T cells to do the exhaustion protocol with good results (I am going to check for exhaustion markers after the stimulation). I have searched papers and they all use untouched CD8+ T cells.
Colt Egelston
Thanks for your reply, that gives me an idea that it will work properly! Anyway, I ordered a kit to positively isolate CD8+ T cells with an additional step to remove the beads. I am going to do a CD8 T cell exhaustion test with the labeled or unlabeled ones.- Badges
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