Basically, many papers say they use 650 and 750 nm light but I wonder if someone measured some for of response curve for intermediate wavelength. (wheter in vitre or in vivo)
I understand these 650 and 750nm don't come from nowhere but still, I don't think such round values are biologically grounded. Also, most standard light sources won't give you exactly a sharp 650nm or 750nm emission... Has anyone published such kind of "wavelength calibration" data ?
We have illuminated our PhyB/PIF6- based gene expression system with light of wavelengths between 650 nm - 760 nm. You can find the data in Fig. 4 of Nat Protoc 9(3):622-632...
Konrad Müller hi, I read your work in plants. Did you observe expression of the system whether in both 650 and 740nm together or white light only? I wonder the system make an expression in solar radiation which both wavelength are quite strong.Article A red light-controlled synthetic gene expression switch for ...
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