I am having a problem during western blot transfer. The proteins and marker are not transfering from gel to nitrocelllulose membrane. Can someone suggest to me the solution.
Hi, what kind of protocol did you use? the problems in your western blot analysis could come from the membrane transferring step.
More details, you can see in the link below that referring to several error which related to the failure of western blot.
http://bitesizebio.com/19799/the-top-10-western-blotting-mistakes-and-solutions/
We need to know a lot more about your protocol and method to answer this. Are you using a wet or dry transfer?
Hope this page is useful for you.
Western Blot: Technique, Theory, and Trouble Shooting
https://lifetein.com/chat/084013-Western-Blot-Technique-Theory-and-Trouble-Shooting
https://lifetein.com/chat/084013-Western-Blot-Technique-Theory-and-Trouble-Shooting
Many factors can affect the transfer. If you are sure of the protocol that you are using, pay attention to the power supply and the voltage in the transfer tank.
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