Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

Does anyone know how to get a scopus or sciencedirect report of the trends in recent years on a specific research topic?

I am trying to obtain a trend report on a specific issue at your instigation in recent years

28 December 2020 848 2 View

Can the content of my research section be shown on the front page?

no details needed

26 December 2020 9,690 3 View

How do you transmit and evaluate ethics and moral values in you teaching activity?

Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?

23 December 2020 5,058 7 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

Does anyone know of cases of complete eye evulsion (eyes jumping out of their sockets) in ancient or medieval sources?

01 November 2020 1,234 3 View

Do you believe SARS-Cov-2 vaccines will yield a robust immune response and memory?

In view of the potential for Covid-19 reinfection and the weak immune response and short duration in patients previously infected with Covid-19, many doubts have emerged if the virus is capable of...

03 October 2020 5,689 7 View

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I'm currently working in a project in which it would be useful to measure: transmural flux, resistence and potencial difference in mucosa biopsies. I've been reading about this method and as far...

23 September 2020 6,976 1 View

mScarlet Elisa Detection?

I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...

20 September 2020 730 2 View

No title

05 August 2020 8,976 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View