.
5% skim mlik blocking 2 hours
1:100 in 5% skim milk + TBS overnight
washing: TBST
I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.
16 July 2024 7,431 1 View
Apart from the size I can´t find any information about the LFP batteries used in BYD ev, for example. Can the size affect the discharge of tha battery or other characteristics?
19 March 2024 2,229 1 View
Con esta pregunta se pretende identificar los métodos y técnicas más adecuadas para abordar las razones por las que las personas drogopendientes totalmente rehabilitadas, se encuentran en...
10 March 2024 6,561 2 View
Hello community, i have trouble degassing Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, any recomendations, previous experience analyzing BET on...
16 January 2024 3,430 1 View
To characterize the electrocatalytic activity of the microelectrodes, the effective surface area was determined using the equation proposed for cylindrical microelectrodes by (Szabo, 1987). But, I...
14 January 2024 8,116 3 View
Hello gaussian users I'm trying to calculate a NMR spectrum of a macrocyclic compound, I was able to optimize the molecule but not able to get the spin-spin couplings. The program shows this...
07 January 2024 3,223 0 View
Is there any relationship between the Austrian theory of business cycles and the bullwhip effect in the supply chain?
05 January 2024 1,754 1 View
The chemical is ethyl chloride also known as chloroethane and the material of the tank is carbon steel. We connect these tanks in series and push the chemical from tank to tank in our transfer...
04 December 2023 6,991 1 View
Hello everyone. I am struggling to get good quality results on my Sanger sequencing reactions. I don't know the specific problem that I am facing. In a single order, most of the chromatograms...
03 November 2023 4,035 0 View
Since retinal is a photosensitive compound, I wonder whether you perform your experiments in darkness or do you allow certain amount of light. I've read some experiments for root development and...
15 October 2023 4,863 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View
Please send me updates regarding the above-mentioned areas in the question mark?
28 July 2024 5,197 3 View