Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Has anybody seen research on Generative Adversarial Networks not image / video / autoencoder based?

Searching for Generative Adversarial Networks applications.

09 October 2020 2,316 2 View

Can the crystallinity of starch or other materials be calculated from the birefringent area?

Hi I would like to know if is it possible to obtain the crystallinity of a material such as starch from the ratio of the birefringent area to total area using the images obtained from light and...

30 March 2020 952 5 View

Why gap opening is fixed in instruments when using cone-plate geometry?

Hello I work with starch gels and I am performing experiments using cone and plate geometry with 1mm of gap because that height allows me to handle the samples better. I still can not understand...

28 March 2019 1,532 8 View

Does Golgi-cox stanning work in tissues storaged in sucrose 30% for 1 month before the stainning?

11 December 2018 5,386 1 View

If I want to do PCR of Cryptosporidium and Giardia to test, is it better to use a nested one?

05 June 2017 2,654 7 View

Can anybody provide some possible methods to dispose heavy metal contaminated soil, specifically Cd contaminated soil for Cocoa Cultivation?

Can anybody provide some possible methods to dispose heavy metal contaminated soil, specifically Cd contaminated soil for Cocoa Cultivation? superficial soil layers (30cm) large land...

02 March 2017 5,722 9 View

What is a good C-ter FLAG-tagged mammalian expression vector?

I need to subclone my GOI in a mammalian expression vector with a C-ter FLAG tag (or a dual FLAG-HA tag) for IP and affinity purification. My protein cannot properly fold with an N-ter tag, hence...

23 October 2016 146 1 View

Where is the problem with bad results on miRNA RT-qPCR?

In our lab we are performing an miRNA isolation from rat liver with Trizol (including the extra step for helping in the recovery of miRNAs) and then we perform the RT reaction with Taqman microRNA...

11 April 2016 3,121 8 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Can't find an ancient zoology paper ?

I'm desperately looking for an old planarian paper which is cited here. Does anyone have an idea how to get hold of it...

21 January 2021 5,957 8 View