I have been trying to block fatty acid oxidation pathways in E.coli BL21 DE3 using Sodium acrylate at a concentration of 0.2 g/L. The carbon source used was octanoic acid (0.4%W/V) supplemented in LB medium. I started my culture, but surprisingly, the cells had not grown and instead they were found to be dead. Could anyone please help me to get rid of this problem . Thanks.

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