I extract RNA from tadpole fat bodies and get 260/230 ratios in the range of 0.5-1.0 while my 260/280 ratios are good (>2). Does anyone have similar experience with fat bodies? I have used ethanol precipitation and RNA cleaning kits, but didn't improve much. Will there be possible downstream effects on qPCR?

I have extracted RNA from brains and tails with equally good 260/230 ratios which means the problem is not due to the protocol but due to the tissue.

Let me know your comments.