Bidisha Paul

5 Questions 2 Answers 0 Followers

Questions related from Bidisha Paul

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

30 July 2024 3,572 1 View

Anyone has optimized conditions for electroporating SKOV3, Caov3, PA-1, and OVCAR3 cell lines with the Neon Transfection system?

I am trying to electroporate SKOV3, Caov3, PA-1, and OVCAR3 cell lines with CRISPR plasmid and sgRNAs. Together the plasmids are about 14kb, hence I have to electroporate. Does anyone have the...

06 May 2024 1,333 0 View

What transfection reagent should I use transfecting HEK293T cells with IVT transcribed sgRNA and plasmid carrying dCas9 backbone?

I am trying to transfect HEK293 cells with dCas9-KRAB-DNMT3A carrying plasmid and multiple IVT sgRNAs. RNAi max invitrogen and Hiperfect Qiagen are only good for small RNAs but not plasmids. Does...

05 October 2023 3,388 1 View

Does RNA extracted from adipose tissue/ fat bodies have low 260/230 ratio in general?

I extract RNA from tadpole fat bodies and get 260/230 ratios in the range of 0.5-1.0 while my 260/280 ratios are good (>2). Does anyone have similar experience with fat bodies? I have used ethanol...

22 May 2020 8,285 0 View

How does leptin influence CRF release in the hypothlamic PVN?

The interaction between leptin and glucocorticoids (GC) is a very dynamic one. Some studies report leptin inhibits HPA axis by supressing CRH release from PVN and directly by reducing GC release...

01 January 1970 1,822 0 View