Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What is difference between In-line transmission (%) and Total transmission (%) value of optically transparent Ceramics?

I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...

20 February 2021 9,405 6 View

Access to supercomputer resources in India?

Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...

15 February 2021 8,264 3 View

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

FLICA assay not working?

Hi everyone, I am trying to perform the FLICA apoptosis assay with my MSCs. However, although the cells look healthy upon inspection and the viability is greater than 90% with cell counting, the...

17 January 2021 6,959 1 View

How to optimize cell suspension protocol for flow in LS-180 cell line?

Hi, I am optimizing a flow protocol for LS-180 cell line, which can not be trypsinized. First experiment wend suboptimal due to detritus and doublets Any trips or tricks for making this cell...

06 January 2021 6,117 2 View

What are these black and white spheres in T25 flask?

I just thawed my 4T1 cancer cells and 24 hours after seeding the cells in T25 flask there were large black and white spheres (approximately 1000times bigger than my cells) in my cell...

18 December 2020 3,302 3 View

Can I fix the cells after H2DCFDA assay for FACS analysis?

Hi ! I'm Yu, and planning to analyze ROS assay with H2DCFDA(Thermo) on MSCs. A lot of protocols instruct that treat H2DCFDA at specific concentration for 5~30 min, 20~30 min of recovery time...

10 December 2020 973 1 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Any expert on Calu-3 cell culture?

I just started working months ago with this peculiar cell line and I still cannot figure out the doubling time or passaging. Some times take 72h to see double amount of seeded cells, some times...

23 October 2020 6,151 2 View

Currently i am culturing Influenza A in MDCK cell line and i wanted to ask is it possible to obtain high titre of virus without adding TPCK trypsin?

I have done 3 blind passages to concentrate the viral stock in the supernatant without adding TPCK Trypsin. After passage 1 my Ct value for INF A was 36.96. After P2 the value decreased to 33.36....

21 October 2020 3,949 3 View