I have a doubt as trypsin can work in the shaking condition, does PNGASE F has a problem in shaking environment such as 200-300 rpm.
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
I want to do wavelet transform of some compounds for their comparative study? Kindly help me in this regard and provide with proper procedure.
31 July 2024 4,170 0 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
How to use energy flexibility in inventory modeling?
29 July 2024 2,192 5 View
Please send me updates regarding the above-mentioned areas in the question mark?
28 July 2024 5,197 3 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
22 July 2024 412 3 View
In breeding trials, environmental variability can significantly affect the results, making it challenging to accurately evaluate the performance of different lines and hybrids.
22 July 2024 3,961 4 View
I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...
26 June 2024 3,664 1 View
Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...
22 June 2024 1,124 5 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
28 May 2024 6,052 2 View
Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...
07 May 2024 4,706 0 View
Hello Please I need urgently a book as reference for trypsine powder dilution to use in tissue culture. With respects
28 April 2024 6,136 0 View
Hello, I am using Trypsin to determine its Km and Vmax values against casein from bovine milk. I know higher enzyme concentrations increase the Vmax value, but does more trypsin also affect the...
04 April 2024 8,608 3 View
Hello, Background: I am trying to find the Vmax and Km values for 0.25% trypsin and the substrate casein. During optimization, I have tried different time points (1-15 minutes) and different...
01 April 2024 1,553 1 View
I am performing fluorogenic assay with trypsin and fluorogenic assay Z-RR-AMC, my readings are decreasing with increasing time. What could be the reason for this strange results. Please...
01 April 2024 4,657 1 View
I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...
30 March 2024 8,491 0 View
I have been trying for months to grow kidney cells from rat kidneys, I tried several methods, serial sieving, cutting and meshing, and using kidney tissue to obtain an outgrowth of cells. I always...
11 March 2024 2,665 1 View