Arun Singh

4 Questions 2 Answers 0 Followers

Questions related from Arun Singh

Does PNGASE F works better in still condition or shaking is the preferred condition?

I have a doubt as trypsin can work in the shaking condition, does PNGASE F has a problem in shaking environment such as 200-300 rpm.

22 November 2022 9,480 0 View

Can I speed vac the methyl amine dissolved in ethanol and dissolve it in water again. Will this work ?

Will methyl amine remain in the vial or it will also get evaporated.

06 May 2022 458 1 View

Why does my in-solution digest precipitates after acidification?

I am using the following reaction condition Thermofisher trypsin dissolved in 50mM acetic acid (1mg/ml): 2 uL Protein sample: 50 ug (dissolved in 50mM ammonium bicarbonate, pH 8) Make the total...

29 April 2022 9,395 3 View

What are the possibility that methanol also precipitates the glycans along with protein ?

In the N-glycomics experiment I am unable to see any glycans nor any protein. I am doubting whether it could be the separation step of protein and N-glycans might be the problem. Actual...

22 March 2022 3,505 3 View