In my study, I am going to observe the cytokine production level in PMA differentiated THP-1 cell after drug treatment.
1. Does PMA itself stimulate cytokine production?
2. if yes, how can i fix this?
thanks in advance.
Yes, PMA itself stimulate cytokine release which is necessary for differentiation.
PMA induces cytokine production, from you description it seems like you want to see the anti-inflammatory potential of your drug, if so, then it's fine to use PMA after that you can treat your cell (however, I would suggest you to use LPS instead in this case, because PMA also induces severe generation of superoxide anions). If you want to see the inflammatory efficacy of your drug (which i guess by your second line "2. if yes, how can i fix this? ") then you should go with you drug only treatment...
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