Hafij Al Mahmud

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What should be the substitution for FBS in cytokine expression profiling?

In my study, I want to see the effect of a drug in mycobacterium tuberculosis induced inflammatory cytokines expression on macrophage cell line. I tried to use both with and without FBS medium but...

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Does PMA (Phorbol-12-myristate-13-acetate) stimulate the cytokine production in THP-1 cell during differentiation?

In my study, I am going to observe the cytokine production level in PMA differentiated THP-1 cell after drug treatment. 1. Does PMA itself stimulate cytokine production? 2. if yes, how can i fix...

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