In a subcutaneous implantation assay (rabbits for example), if there are no signs of inflammatory reaction upon explantation and histology, does it mean that the material is non pyrogenic as well ? What are the other minor detectable signs of pyrogenecity, other than fever ? Thanks!
I don't believe you can make that inference. Pyrogenicity testing and in vivo histology are different, for implant safety testing purposes.
Inflammatory reaction can be acute or chronic. One can recognize the different cell types in the implant vicinity, hence pyrogenicity is detected by raising the local temperature or even worse by raised systemic temperature.
There can be inflammation without raising the temperature and there can be raised temperature without morphological signs of inflammation.
IL1b levels in the serum/plasma and/or in an extract of the tissue surrounding the implant could serve as marker for pyrogenicity.
Easier is with measuring the temperature...
Cheers,
Ivan
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