How to zero IRGA in Licor6400-XT when CO2 sample is not close to 0 ?

I am doing Zero IRGA now, but the CO2 concentration in the chamber (CO2-S) is maintained around 20. It says the soda lime needs to be changed in the manual, however, after I changed it, it is...

09 October 2016 8,014 9 View

How can I explain the data when ANOVA test results are different from the post hoc analysis results?

01 February 2016 1,579 9 View

How can I analyse non-normal distributed data?

I have a three-factorial experiment, and I am going to use three-way ANOVA, however, some of the response variables dot not fit in the assumption of normality (I use residuals), no matter how I...

11 December 2014 628 16 View

ANCOVA or repeated measures ANOVA?

08 September 2014 9,328 8 View

Do any other free sugars have a bigger molecule size than sucrose in perennial ryegrass?

I ran my samples in HPLC with a carbohydrate analysis column HPX-87C, with water as mobile phase, flow rate 0.6 ml/s, to determine the content of sucrose, glucose and fructose. However, there is...

07 August 2014 1,932 3 View

What is the difference between the general linear model (GLM)and generalized linear model (GZLM)?

I used to think they were the same thing until today, I found out they are now different.

04 May 2014 7,221 12 View

What kind of bags can I use to put plant samples in and then subject to freeze-drying?

I put leaf samples in the plastic seal bags, and freeze-dried, but the majority of the bags were broken and some samples were lost. Anyone have experience with this?

04 May 2014 3,317 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View