Does MC38 Mouse Adenocarcinoma Cell lines have G13R mutation in KRAS Gene?

12 December 2017 1 2K Report

I got some MC38 from a fellow researcher. I am focusing on G13R mutation. Literature suggests that this cell line is mutated on 13th animo acid. But, I sequenced the sample and found no mutation in this cell line.

Could anyone tell me where i went wrong? Did i get a cell line without G13 mutation? or MC38 cell line might not have that mutation?

Thanks in advance.

Yingjie Li

You should trust your sequenced results that MC38 cells expressing WT KRAS, the results consisted with the recent references [BMC Genomics 21, 2 (2020). https://doi.org/10.1186/s12864-019-6344-3] [Clin Cancer Res. 2021 Feb 1;27(3):865-876. doi: 10.1158/1078-0432.CCR-20-2385.]

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