Does Hydration time affect Liposome size Production?

Maria Isabel Dumlao @Maria-Isabel-Dumlao-2

13 July 2020 1 10K Report

Does prolonged hydration time affect the liposome size production? Our hydration step is followed with a sonication step, and the diameter size of the liposomes do not reach ~300nm. I have observed in our plant-scale set-up, the hydration time is longer than 1hr for a batch size of 200litres

Gürhan Abuhanoğlu

Hydration time can affect particle size. Especially if you are working at a high volume. The addition of small glass beads during hydration affects hydration and longer duration and allows more optimized liposomes to be obtained.

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