Hello, everyone!
Does anybody know whether horse serum has platelet activating factor (PAF) or not? I treated cells with wortmannin and I could see that this PI3K inhibitor was able to inactivate MAPK. I looked on literature and there is a study about this effect, but it is dependent on PAF. I also looked the composition of the medium (DMEM/F12) I used to seed cells, but it seems it doesn´t have PAF.
Can anybody help me?
Thanks a lot!
Angélica: It is likely that horse serum, like sera from other species, contains PAF released from blood cells (macrophages) . Its a matter of how much PAF in different sera. One way to eliminate possible PAF from sera is to use serum free DMEM/F12 briefly by adding ITS (insulin, transferrin, and selenium) prior to and during your Wortmannin studies. Good luck.
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