01 April 2015 2 791 Report

Does homologous recombination occur in mouse cells, specifically NIH-3T3 cells (fibroblasts)?  I was wondering if you make a monoclonal heterozygote where a gene is KO in one chromosome but not the other, if this would be a stable system?  I am specifically asking for CRISPR/Cas but if anyone has experience with monoclonal mouse heterozygote cell lines, I am sure the same principle applies. Thanks!

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If using polymers for flocculant in a waste water treatment plant how often should they have a tox test done?

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After working tirelessly with your graduate student to carry out a research and making serous input in a journal article involving both of you., who should be the first author of this publication?

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