Does heavy granulation indicates Mycoplasma contamination in cell lines?

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Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

Can I publish research with researchgate?

Can I publish paper with researchgate?

19 May 2024 698 1 View

What are the data required to assess the impact of climate change on agriculture and food security?

all relevant data required in conducting this research.

10 March 2024 7,026 3 View

Gerber file extraction in CST?

I have a feed alone as one of the layer. When i try to extract that, I get the folloing error. Kindly help me , how to resolve the issue and extract the gerber file

29 February 2024 3,772 2 View

What is the best way to photobleach eGFP?

Dear all, I'm doing cell culture with lentiviruses including eGFP. While this is a very useful way to check transduction efficiency, it does take up a channel with subsequent immunofluorescence...

20 February 2024 1,660 2 View

Common scenario, experimental vignette and response to a question?

I want respondents to see a scenario (common scenario that contain uncivilized behavior of consumers) and then provide vignette carrying a message (manipulative variables-Proscriptive Vs...

04 February 2024 4,952 0 View

What is the best far-red dye for fixed cells?

Dear all, We have a Olympus FV1200 confocal microscope with 405, 473, 559, and 635 lasers. I'm looking for a nuclear dye (fixed cells) in the far red range, does anyone have any recommendations...

23 January 2024 6,491 4 View

Please advise theoretical aspect of business management in construction ?

22 January 2024 567 3 View

How to add survey data to a map in ArcMap GIS?

Hello, I have data that I collected from a survey that contains qualitative data (ordinal/nominal) and includes the participant’s residential neighborhood, 800 participants distributed over 50...

24 December 2023 8,001 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View