Does FuDR dissolve/dilute into NGM plates when administered to plates?

28 August 2020 1 2K Report

I am currently trying to use FuDR to run a lifespan experiment using C. elegans.

I have a stock solution of FuDR that has been dissolved in water and am trying the determine the concentration of FuDR once it is administered to NGM plates. My question is whether or not the FuDR will dissolve into the plates to dilute the concentration, or if it will simply deposit on the surface.

Thanks!

Lucas Mergan

In our lab we always calculate the final concentration based on the total volume of the plates, in the expectation that it spreads throughout the entire plate over time. However, I am not sure if this has been tested or if it is just an assumption.

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