Lucas Mergan

3 Questions 8 Answers 0 Followers

Questions related from Lucas Mergan

Are biotinylated proteins stable at -80 °C?

I would like to store biological samples containing biotinylated proteins at -80 °C for several weeks, before continuing protein extraction and streptavidin affinity purification. I was wondering...

15 June 2020 4,036 6 View

C. elegans UV integration troubleshooting?

I have been trying to integrate a simple unc-122p::GFP array after microinjection by using the protocol on WormBook. I have repeated this twice, each time singling around 150 fluorescent progeny...

05 July 2019 5,993 1 View

Approximately how many cells are needed for RNC sequencing?

I intended to perform polysome sequencing upon FAC-sorted C. elegans cells, but the required number of cells is not achievable for my application. Ribosome nascent-chain complex (RNC) sequencing...

05 June 2019 7,649 1 View