Does fluorescence have an influence on hyperspectral imgaing? Is the fluorescence too weak compared to the incident light?
In general, fluorescence is too weak to affect any kind of imaging that does not filter out incident light. High intensity voxels in typical high resolution fluorescence confocal microscopy are based on about 10 emitted photons, for instance. If you have incident light with overlapping wavelengths, you usually get reflected and scattered photon numbers far above this.
If you use an incident light that has a high intensity spectral peak at the absorption peak of a fluorophore and has minimal intensity elsewhere along the observed spectral region, you will of course mainly observe fluorescence emission, but this is usually simply fluorescence imaging.
If you look at the Quantum Efficiency of typical fluorescent dyes you will get an idea which influence it could have. Usually it in the range of single percent or less if excited at perfect wavelength. If you calculate which part of the spectrum of yoh incident light might excite fluorescence, you can get an quite good estimation of the influence.
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