Does flow cytometry detect perforin and granzyme B in T cells need stimulation?

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Jitendra Kumar Shandilya

Dear Qing Xiao

You can check perforin and granzyme on the resting cells as well. We did not stimulate blood in our experiments and found good results. Even in clinical diagnosis of HLH at our centre, we do not stimulate cells for Perforin.

Those who stimulated cells (which you read) might also be checking de-granulations (CD107a) study altogether. And CD107a expression needs stimulations.

Please find attached link of abstract from our centre.

https://academic.oup.com/rheumatology/article/57/suppl_3/key075.201/4971101

Best wishes.

Qing Xiao

Jitendra Kumar Shandilya Thanks for your valuable answer, and it was very beneficial for our research. We have studied your research thoroughly, and your approach will be applied in our future studies. Many thanks!