Does DMSO can have cytotoxic effects on microorganisms and may interfere with the results of microbiological assays?

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

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What is the most suitable medium of ascomycetes fungi ??

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Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

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A.B Bayazid

Dear Saad Al-Arnoot

Yes, it could be cytotoxic depending on its concentration and less than 1% is mainly recommended in a working solution.

Article The effect of organic solvents on selected microorganisms an...

Article Sodium butyrate ameliorates neurotoxicity and exerts anti-in...

https://www.sciencedirect.com/science/article/abs/pii/S0022354915369057

Erica L Suchman

When DMSO is added to culture medium to freeze eukaryotic cell lines, and the cells are thawed, DMSO must be removed within a few hours or the cells will die. Although DMSO helps stabilize cells during freezing, it does have toxicity, so the amount of time it is left in contact is important, as well as the concentration as noted in the last comment.

Amira Al-harethi

Damso cannot be used to research microbial activities since it is thought to be lethal to it. Many studies suggest using 10 microliters of it in addition to 990 milliliters of distilled water to produce 1 ml.

Good Luck Saad