How many tumor cells should I seed in a 96-well plate/well for virus infectivity?

The tumor cells are from a variety of cell lines from ATCC .

22 December 2020 1,950 3 View

How does band gap of a semiconductor shift with the lattice strain? and physical significance?

Someone posted a similar question but I have a further question on strain (increase/ decrease atom distance). So I know that the distance increases and decreases the bandgap distance. But can it...

04 June 2020 689 5 View

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance and yield attributing traits self pollinat?

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance in self pollinating crops (F2 generation) Vs cross pollinating crops, is it important to...

30 March 2020 1,011 3 View

What is the most appropriate statistical test for my experiment?

I am having trouble identifying the most appropriate statistical test for an experiment I am designing. In this experiment I am looking at multiple markers that indicate a change in structure...

25 November 2019 9,090 2 View

Where could I find some SOPs and formulations for making in house Clinical chemistry reagents?

Hi, I am looking for some resources to be able to find some SOPs and formulations for making in house Clinical enzymatic chemistry reagents like; Triglyceride, HDL-Cholestrol, Oxalate, Lactate,...

25 July 2019 698 2 View

Has anyone else had any problems with PSA antibodies for WB?

I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots....

14 July 2019 2,723 3 View

When thawing cryopreserved cells, can they be added to 0.9% NaCl without loss of viability?

The cells are cryopreserved in 8% DMSO and 2% HSA.

08 November 2018 3,807 3 View

What is best way to check for non-proliferation in a tumor cell lysate?

We use 6x freeze-thaw cycles and consistently show 0% viability by Trypan Blue. We have used MTT and results were good, but some say DNA assays are better. Thanks

03 August 2018 4,119 3 View

What is second order CFA?

Hi ? I ran CFA in AMOS and fit indices were poor . I ran EFA in SPSS (etracted six factors) and delete some Items that were cross-loading , then run CFA again and now fit indices are within...

30 July 2018 9,679 5 View

Any antibodies against MMP2 and MMP9 that are suitable immunohisto?

Hello All. Can anyone suggest antibodies against MMP2 and MMP9 that work well for immunohisto in the mouse heart? Thanks in advance. Bruce

24 November 2016 7,284 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Can HIV drugs will be a provisional or recurring option against COVID-19?

Can HIV drugs will be a provisional or recurring option against COVID-19?

22 December 2020 7,872 2 View

Can the formation of drugs against coronavirus also be useful against the Influenzas virus or HIV?

Can the formation of drugs against coronavirus also be useful against the Influenzas virus or HIV? This is because these viruses shared many common proteases for viral replication.

22 December 2020 6,508 6 View

Why accuracy over each k-fold corssValidation differ alot?

I have a data set with 91 Obervsation with 700 features. I have reduced the dimension of data using PCA? then I split the data set into 70/30. After that applied kfold =10 cross-validations on...

04 December 2020 1,259 4 View

What is expected from a PhD ?

PhD is considered as the highest degree one can get from the university and also is recognized worldwide. What is expected out of a PhD degree, the holder and the providing institutes ? What are...

11 October 2020 9,412 13 View

Please some paper about Adaptive immunity against viral infection???

26 April 2020 1,999 10 View

Can someone send me the list of RNA dependent RNA polymerase inhibitors?

It would be very helpful if someone can send me a link or text containing the list RNA dependent RNA POLYMERASE (RdRp) inhibitors.

20 March 2020 5,707 4 View

What is the appropriate statistical treatment to be used if our study aims to identify the level of knowledge of college students with regards to HIV?

We will use a survey which contains close-ended questions.

14 March 2020 9,676 3 View

How would I determine if a protein (PD-1) is necessary for establishment of latent infection by a virus in a CD4 memory cell line?

If I were to plan an experiment to deduce whether or not PD-1 is absolutely necessary for HIV to establish latent infection in CD4 memory cells, how would I go about doing this? More...

09 March 2020 7,774 2 View