There is a significant peak shift observed in the CD spectra of BSA during drug binding which may be indicative of denaturation which may affect its ability to carry this drug, but the results of FTIR, synchronous fluorescence and molecular dynamic simulations indicate that the drug binding does not induce conformational instabilty to protein backbone as not much changes were observed. Please help me to explain the experimental findings of circular dichroism studies.

Thank you in advance.